› 2wk6

X-ray diffraction
2.5Å resolution

Structural features of native human thymidine phosphorylase and in complex with 5-iodouracil

Released:
DOI: 10.2210/pdb2wk6/pdb
PDB entry 2wk6 coloured by chain, front view.
PDB entry 2wk6 coloured by chain, side view.
PDB entry 2wk6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of native human thymidine phosphorylase and in complex with 5-iodouracil.
Mitsiki E, Papageorgiou AC, Iyer S, Thiyagarajan N, Prior SH, Sleep D, Finnis C, Acharya KR
Biochem Biophys Res Commun 386 666-70 (2009)
PMID: 19555658

Function and Biology Details

Reaction catalysed: 
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 50 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19971 (Residues: 1-482; Coverage: 100%)
Gene names: ECGF1, TYMP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IUR 2 x IUR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 61.911Å b: 67.266Å c: 212.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.283
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Thymidine phosphorylase: A potential new target for treating cardiovascular disease.
Li et al. (2018)
14 other articles

3 mentions without citation

Structures of native human thymidine phosphorylase and in complex with 5-iodouracil.
Mitsiki et al. (2009)
2 more