› 2wo6

X-ray diffraction
2.5Å resolution

Human Dual-Specificity Tyrosine-Phosphorylation-Regulated Kinase 1A in complex with a consensus substrate peptide

Released:
DOI: 10.2210/pdb2wo6/pdb
PDB entry 2wo6 coloured by chain, front view.
PDB entry 2wo6 coloured by chain, side view.
PDB entry 2wo6 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Down syndrome kinases, DYRKs, reveal mechanisms of kinase activation and substrate recognition.
Soundararajan M, Roos AK, Savitsky P, Filippakopoulos P, Kettenbach AN, Olsen JV, Gerber SA, Eswaran J, Knapp S, Elkins JM
Structure 21 986-96 (2013)
PMID: 23665168

Function and Biology Details

Reactions catalysed: 
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-171791 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity tyrosine-phosphorylation-regulated kinase 1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 44.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13627 (Residues: 127-485; Coverage: 47%)
Gene names: DYRK, DYRK1A, MNB, MNBH
Sequence domains: Protein kinase domain
Structure domains:
Protein crumbs homolog 2 Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 783 Da
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q5IJ48 (Residues: 4-9; Coverage: 0%)
Gene name: CRB2

Ligands and Environments

2 bound ligands:
Ligand D15 2 x D15
Ligand CL 1 x CL
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P65
Unit cell:
a: 168.43Å b: 168.43Å c: 62.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

DYRK1A, a Dosage-Sensitive Gene Involved in Neurodevelopmental Disorders, Is a Target for Drug Development in Down Syndrome.
Duchon et al. (2016)
16 more

22 mentions without citation

JNK Signaling: Regulation and Functions Based on Complex Protein-Protein Partnerships.
Zeke et al. (2016)
21 more