› 2wp2

X-ray diffraction
2.37Å resolution

Structure of Brdt bromodomain BD1 bound to a diacetylated histone H4 peptide.

Released:
DOI: 10.2210/pdb2wp2/pdb
PDB entry 2wp2 coloured by chain, front view.
PDB entry 2wp2 coloured by chain, side view.
PDB entry 2wp2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cooperative binding of two acetylation marks on a histone tail by a single bromodomain.
Morinière J, Rousseaux S, Steuerwald U, Soler-López M, Curtet S, Vitte AL, Govin J, Gaucher J, Sadoul K, Hart DJ, Krijgsveld J, Khochbin S, Müller CW, Petosa C
Nature 461 664-8 (2009)
PMID: 19794495

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158701 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain testis-specific protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 14.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91Y44 (Residues: 17-136; Coverage: 13%)
Gene names: Brdt, Fsrg3
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H4 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 20 amino acids
Theoretical weight: 2.08 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P62806 (Residues: 2-21; Coverage: 19%)
Gene names: H4-12, H4-53, H4c1, H4c11, H4c12, H4c14, H4c16, H4c2, H4c3, H4c4, H4c6, H4c8, H4c9, H4f16, Hist1h4a, Hist1h4b, Hist1h4c, Hist1h4d, Hist1h4f, Hist1h4h, Hist1h4i, Hist1h4j, Hist1h4k, Hist1h4m, Hist2h4, Hist2h4a, Hist4h4

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 4 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 49.293Å b: 61.224Å c: 97.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.261
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

97 review citations

Targeting bromodomains: epigenetic readers of lysine acetylation.
Filippakopoulos et al. (2014)
96 more

13 mentions without citation

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
12 more