2wss

X-ray diffraction
3.2Å resolution

The structure of the membrane extrinsic region of bovine ATP synthase

Released:
Source organism: Bos taurus
Primary publication:
The structure of the membrane extrinsic region of bovine ATP synthase.
Proc Natl Acad Sci U S A 106 21597-601 (2009)
PMID: 19995987

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tridecamer (preferred)
hetero dodecamer
PDBe Complex ID:
PDB-CPX-133619 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C, J, K, L
ATP synthase subunit beta, mitochondrial Chains: D, E, F, M, N, O
Molecule details ›
Chains: D, E, F, M, N, O
Length: 482 amino acids
Theoretical weight: 51.76 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 47-528; Coverage: 91%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
Structure domains:
ATP synthase subunit gamma, mitochondrial Chains: G, P
Molecule details ›
Chains: G, P
Length: 272 amino acids
Theoretical weight: 30.19 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 26-297; Coverage: 91%)
Gene names: ATP5C, ATP5C1, ATP5F1C
Sequence domains: ATP synthase
Structure domains:
ATP synthase subunit delta, mitochondrial Chains: H, Q
Molecule details ›
Chains: H, Q
Length: 146 amino acids
Theoretical weight: 15.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05630 (Residues: 23-168; Coverage: 87%)
Gene names: ATP5D, ATP5F1D
Sequence domains:
Structure domains:
ATP synthase subunit epsilon, mitochondrial Chains: I, R
Molecule details ›
Chains: I, R
Length: 50 amino acids
Theoretical weight: 5.66 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P05632 (Residues: 2-51; Coverage: 98%)
Gene names: ATP5E, ATP5F1E
Sequence domains: Mitochondrial ATP synthase epsilon chain
Structure domains: ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial
ATP synthase subunit O, mitochondrial Chains: S, W
Molecule details ›
Chains: S, W
Length: 190 amino acids
Theoretical weight: 21.36 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13621 (Residues: 24-213; Coverage: 89%)
Gene names: ATP5O, ATP5PO
Sequence domains: ATP synthase delta (OSCP) subunit
Structure domains: N-terminal domain of the delta subunit of the F1F0-ATP synthase
ATP synthase F(0) complex subunit B1, mitochondrial Chains: T, X
Molecule details ›
Chains: T, X
Length: 116 amino acids
Theoretical weight: 14.11 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13619 (Residues: 141-256; Coverage: 45%)
Gene names: ATP5F, ATP5F1, ATP5PB
Sequence domains: Mitochondrial ATP synthase B chain precursor (ATP-synt_B)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
ATP synthase subunit d, mitochondrial Chain: U
Molecule details ›
Chain: U
Length: 118 amino acids
Theoretical weight: 13.39 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13620 (Residues: 2-119; Coverage: 73%)
Gene names: ATP5H, ATP5PD
Sequence domains: ATP synthase D chain, mitochondrial (ATP5H)
ATP synthase-coupling factor 6, mitochondrial Chains: V, Z
Molecule details ›
Chains: V, Z
Length: 76 amino acids
Theoretical weight: 9.06 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P02721 (Residues: 33-108; Coverage: 70%)
Gene names: ATP5J, ATP5PF
Sequence domains: Mitochondrial ATP synthase coupling factor 6
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 158.244Å b: 231.245Å c: 286.361Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.271
Expression systems:
  • Not provided
  • Escherichia coli BL21