› 2wub

X-ray diffraction
2.9Å resolution

Crystal structure of HGFA in complex with the allosteric non- inhibitory antibody Fab40.deltaTrp

Released:
DOI: 10.2210/pdb2wub/pdb
PDB entry 2wub coloured by chain, front view.
PDB entry 2wub coloured by chain, side view.
PDB entry 2wub coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unraveling the allosteric mechanism of serine protease inhibition by an antibody.
Ganesan R, Eigenbrot C, Wu Y, Liang WC, Shia S, Lipari MT, Kirchhofer D
Structure 17 1614-1624 (2009)
PMID: 20004165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211910 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hepatocyte growth factor activator long chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 257 amino acids
Theoretical weight: 27.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q04756 (Residues: 408-655; Coverage: 40%)
Gene name: HGFAC
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hepatocyte growth factor activator short chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 35 amino acids
Theoretical weight: 3.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q04756 (Residues: 373-407; Coverage: 6%)
Gene name: HGFAC
FAB FRAGMENT FAB40.DELTATRP HEAVY CHAIN Chains: H, R
Molecule details ›
Chains: H, R
Length: 224 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB FRAGMENT FAB40.DELTATRP LIGHT CHAIN Chains: L, Q
Molecule details ›
Chains: L, Q
Length: 214 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 72.356Å b: 89.53Å c: 118.47Å
α: 90° β: 91.08° γ: 90°
R-values:
R R work R free
0.216 0.216 0.275
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

8 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
7 more

2 mentions without citation

Allostery and population shift in drug discovery.
Kar et al. (2010)
1 more