› 2wuc

X-ray diffraction
2.7Å resolution

Crystal structure of HGFA in complex with the allosteric non- inhibitory antibody Fab40.deltaTrp and Ac-KQLR-chloromethylketone

Released:
DOI: 10.2210/pdb2wuc/pdb
PDB entry 2wuc coloured by chain, front view.
PDB entry 2wuc coloured by chain, side view.
PDB entry 2wuc coloured by chain, top view.
Source organisms:
Primary publication:
Unraveling the allosteric mechanism of serine protease inhibition by an antibody.
Ganesan R, Eigenbrot C, Wu Y, Liang WC, Shia S, Lipari MT, Kirchhofer D
Structure 17 1614-1624 (2009)
PMID: 20004165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211608 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Hepatocyte growth factor activator long chain Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 27.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q04756 (Residues: 408-655; Coverage: 40%)
Gene name: HGFAC
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hepatocyte growth factor activator short chain Chain: B
Molecule details ›
Chain: B
Length: 35 amino acids
Theoretical weight: 3.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q04756 (Residues: 373-407; Coverage: 6%)
Gene name: HGFAC
FAB FRAGMENT FAB40.DELTATRP HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
ACE-KQLR-CHLOROMETHYLKETONE INHIBITOR Chain: I
Molecule details ›
Chain: I
Length: 6 amino acids
Theoretical weight: 606 Da
Source organism: synthetic construct
Expression system: Not provided
FAB FRAGMENT FAB40.DELTATRP LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 80.359Å b: 147.889Å c: 146.371Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.274
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
8 more

6 mentions without citation

AbDesign: An algorithm for combinatorial backbone design guided by natural conformations and sequences.
Lapidoth et al. (2015)
5 more