› 2wz1

X-ray diffraction
1.63Å resolution

STRUCTURE OF THE CATALYTIC DOMAIN OF HUMAN SOLUBLE GUANYLATE CYCLASE 1 BETA 3.

Released:
DOI: 10.2210/pdb2wz1/pdb
PDB entry 2wz1 coloured by chain, front view.
PDB entry 2wz1 coloured by chain, side view.
PDB entry 2wz1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the catalytic domain of human soluble guanylate cyclase.
Allerston CK, von Delft F, Gileadi O
PLoS One 8 e57644 (2013)
PMID: 23505436

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate cyclase soluble subunit beta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02153 (Residues: 408-619; Coverage: 34%)
Gene names: GUC1B3, GUCSB3, GUCY1B1, GUCY1B3
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 65.59Å b: 90.38Å c: 140.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

The chemistry and biology of soluble guanylate cyclase stimulators and activators.
Follmann et al. (2013)
20 more

8 mentions without citation

Structure/function of the soluble guanylyl cyclase catalytic domain.
Childers et al. (2018)
7 more