› 2x1v

X-ray diffraction
1.7Å resolution

Crystal Structure of the activating H-Ras I163F mutant in Costello Syndrome, bound to MG-GDP

Released:
DOI: 10.2210/pdb2x1v/pdb
PDB entry 2x1v coloured by chain, front view.
PDB entry 2x1v coloured by chain, side view.
PDB entry 2x1v coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Anand K

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P63
Unit cell:
a: 73.14Å b: 73.14Å c: 62.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.227
Expression system: Escherichia coli

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