› 2x2e

X-ray diffraction
2Å resolution

Dynamin GTPase dimer, long axis form

Released:
DOI: 10.2210/pdb2x2e/pdb
PDB entry 2x2e coloured by chain, front view.
PDB entry 2x2e coloured by chain, side view.
PDB entry 2x2e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
G domain dimerization controls dynamin's assembly-stimulated GTPase activity.
Chappie JS, Acharya S, Leonard M, Schmid SL, Dyda F
Nature 465 435-40 (2010)
PMID: 20428113

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dynamin-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 353 amino acids
Theoretical weight: 39.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05193 (Residues: 6-320, 726-750; Coverage: 39%)
Gene names: DNM, DNM1
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GDP 2 x GDP
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand NA 2 x NA
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 43.61Å b: 81.02Å c: 181.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

46 review citations

Dynamin, a membrane-remodelling GTPase.
Ferguson et al. (2012)
45 more

30 mentions without citation

Membrane fission by dynamin: what we know and what we need to know.
Antonny et al. (2016)
29 more