› 2x35

X-ray diffraction
2Å resolution

Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.

Released:
DOI: 10.2210/pdb2x35/pdb
PDB entry 2x35 coloured by chain, front view.
PDB entry 2x35 coloured by chain, side view.
PDB entry 2x35 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of histone H3K36me3 recognition by the PWWP domain of Brpf1.
Vezzoli A, Bonadies N, Allen MD, Freund SM, Santiveri CM, Kvinlaug BT, Huntly BJ, Göttgens B, Bycroft M
Nat Struct Mol Biol 17 617-9 (2010)
PMID: 20400950

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peregrin Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55201 (Residues: 1076-1205; Coverage: 11%)
Gene names: BR140, BRPF1
Sequence domains: PWWP domain
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 44.214Å b: 62.399Å c: 114.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

44 review citations

Understanding the language of Lys36 methylation at histone H3.
Wagner et al. (2012)
43 more

2 mentions without citation

PWWP domains and their modes of sensing DNA and histone methylated lysines.
Rona et al. (2016)
1 more