› 2x6g

X-ray diffraction
2.18Å resolution

X-ray Structure of Macrophage Inflammatory Protein-1 alpha (D27A)

Released:
DOI: 10.2210/pdb2x6g/pdb
PDB entry 2x6g coloured by chain, front view.
PDB entry 2x6g coloured by chain, side view.
PDB entry 2x6g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme.
Ren M, Guo Q, Guo L, Lenz M, Qian F, Koenen RR, Xu H, Schilling AB, Weber C, Ye RD, Dinner AR, Tang WJ
EMBO J 29 3952-66 (2010)
PMID: 20959807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-C motif chemokine 3 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Length: 70 amino acids
Theoretical weight: 7.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10147 (Residues: 23-92; Coverage: 100%)
Gene names: CCL3, G0S19-1, MIP1A, SCYA3
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 57.211Å b: 113.527Å c: 173.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.21 0.286
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Chemokine oligomerization and interactions with receptors and glycosaminoglycans: the role of structural dynamics in function.
Salanga et al. (2011)
20 more

7 mentions without citation

Stuck in the middle: structural insights into the role of the gH/gL heterodimer in herpesvirus entry.
Stampfer et al. (2013)
6 more