› 2x72

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF THE CONSTITUTIVELY ACTIVE E113Q,D2C,D282C RHODOPSIN MUTANT WITH BOUND GALPHACT PEPTIDE.

Released:
DOI: 10.2210/pdb2x72/pdb
PDB entry 2x72 coloured by chain, front view.
PDB entry 2x72 coloured by chain, side view.
PDB entry 2x72 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The structural basis of agonist-induced activation in constitutively active rhodopsin.
Standfuss J, Edwards PC, D'Antona A, Fransen M, Xie G, Oprian DD, Schertler GF
Nature 471 656-60 (2011)
PMID: 21389983

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.27 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 340-350; Coverage: 3%)
Gene name: GNAT1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
6 bound ligands:
Ligand PEF 1 x PEF
Ligand BOG 1 x BOG
Ligand PLM 2 x PLM
Ligand RET 1 x RET
Ligand LPP 1 x LPP
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: R32
Unit cell:
a: 243.986Å b: 243.986Å c: 109.156Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.244
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

75 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
74 more

38 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
37 more