› 2xa7

X-ray diffraction
3.1Å resolution

AP2 clathrin adaptor core in active complex with cargo peptides

Released:
DOI: 10.2210/pdb2xa7/pdb
PDB entry 2xa7 coloured by chain, front view.
PDB entry 2xa7 coloured by chain, side view.
PDB entry 2xa7 coloured by chain, top view.
Source organisms:
Primary publication:
A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex.
Jackson LP, Kelly BT, McCoy AJ, Gaffry T, James LC, Collins BM, Höning S, Evans PR, Owen DJ
Cell 141 1220-9 (2010)
PMID: 20603002

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero tetramer
monomeric
PDBe Complex ID:
PDB-CPX-148383 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 69.66 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18484 (Residues: 1-271, 272-620; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 592 amino acids
Theoretical weight: 67.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63010 (Residues: 1-592; Coverage: 63%)
Gene names: ADTB2, AP2B1, CLAPB1
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 446 amino acids
Theoretical weight: 51.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84092 (Residues: 1-236, 237-435; Coverage: 100%)
Gene name: Ap2m1
Sequence domains:
Structure domains: Beta-Lactamase
TGN38 CARGO PEPTIDE Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 809 Da
Source organism: Homo sapiens
Expression system: Not provided
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62743 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 255.316Å b: 255.316Å c: 156.754Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.236 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

52 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
51 more

28 mentions without citation

Molecular structure, function, and dynamics of clathrin-mediated membrane traffic.
Kirchhausen et al. (2014)
27 more