› 2xgy

X-ray diffraction
1.8Å resolution

Complex of Rabbit Endogenous Lentivirus (RELIK)Capsid with Cyclophilin A

Released:
DOI: 10.2210/pdb2xgy/pdb
PDB entry 2xgy coloured by chain, front view.
PDB entry 2xgy coloured by chain, side view.
PDB entry 2xgy coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analysis of prehistoric lentiviruses uncovers an ancient molecular interface.
Goldstone DC, Yap MW, Robertson LE, Haire LF, Taylor WR, Katzourakis A, Stoye JP, Taylor IA
Cell Host Microbe 8 248-59 (2010)
PMID: 20833376

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158790 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RELIK CAPSID N-TERMINAL DOMAIN Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.51 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Human Immunodeficiency Virus Type 1 Capsid Protein
Peptidyl-prolyl cis-trans isomerase A Chain: B
Molecule details ›
Chain: B
Length: 173 amino acids
Theoretical weight: 18.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62937 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 117.158Å b: 117.158Å c: 49.655Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Endogenous viruses: insights into viral evolution and impact on host biology.
Feschotte et al. (2012)
12 more

1 mention without citation

Interface residues of transient protein-protein complexes have extensive intra-protein interactions apart from inter-protein interactions.
Jayashree et al. (2019)