› 2xh1

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reactions catalysed: 
L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate
Glycine + 2-oxoglutarate = glyoxylate + L-glutamate
(1a) L-kynurenine + glyoxylate = 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine 
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
L-methionine + glyoxylate = 4-methylthio-2-oxobutanoate + glycine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 425 amino acids
Theoretical weight: 47.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N5Z0 (Residues: 1-425; Coverage: 100%)
Gene names: AADAT, KAT2, KYAT2
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand BF5 2 x BF5
Ligand IOD 1 x IOD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 98.323Å b: 152.857Å c: 60.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The kynurenine pathway as a therapeutic target in cognitive and neurodegenerative disorders.
Stone et al. (2013)
1 more

5 mentions without citation

Major Developments in the Design of Inhibitors along the Kynurenine Pathway.
Jacobs et al. (2017)
4 more