› 2xhi

X-ray diffraction
1.55Å resolution

Separation-of-function mutants unravel the dual reaction mode of human 8-oxoguanine DNA glycosylase

Released:
DOI: 10.2210/pdb2xhi/pdb
PDB entry 2xhi coloured by chain, front view.
PDB entry 2xhi coloured by chain, side view.
PDB entry 2xhi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Separation-of-function mutants unravel the dual-reaction mode of human 8-oxoguanine DNA glycosylase.
Dalhus B, Forsbring M, Helle IH, Vik ES, Forstrøm RJ, Backe PH, Alseth I, Bjørås M
Structure 19 117-27 (2011)
PMID: 21220122

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115932 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
N-glycosylase/DNA lyase Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 40.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15527 (Residues: 1-345; Coverage: 100%)
Gene names: MMH, MUTM, OGG1, OGH1
Sequence domains:
Structure domains:
5'-D(*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*CP*GP*TP*CP*CP*AP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.56 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand 8OG 1 x 8OG

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P6522
Unit cell:
a: 92.669Å b: 92.669Å c: 210.986Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.265
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

8-Oxoguanine DNA glycosylase 1: Beyond repair of the oxidatively modified base lesions.
Ba et al. (2018)
9 more

9 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
8 more