› 2xph

X-ray diffraction
2.4Å resolution

Crystal structure of human SENP1 with the bound cobalt

Released:
DOI: 10.2210/pdb2xph/pdb
PDB entry 2xph coloured by chain, front view.
PDB entry 2xph coloured by chain, side view.
PDB entry 2xph coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The role of Co²+ in the crystallization of human SENP1 and comments on the limitations of automated refinement protocols.
Rimsa V, Eadsforth T, Hunter WN
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 442-5 (2011)
PMID: 21505236

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sentrin-specific protease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P0U3 (Residues: 415-644; Coverage: 36%)
Gene name: SENP1
Sequence domains: Ulp1 protease family, C-terminal catalytic domain
Structure domains: Adenoviral Proteinase; Chain A

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 71.172Å b: 71.172Å c: 199.988Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.227 0.313
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form.
Baum et al. (2015)

40 mentions without citation

Advances in the development of SUMO specific protease (SENP) inhibitors.
Kumar et al. (2015)
39 more