› 2xqb

X-ray diffraction
2.6Å resolution

Crystal Structure of anti-IL-15 Antibody in Complex with human IL-15

Released:
DOI: 10.2210/pdb2xqb/pdb
PDB entry 2xqb coloured by chain, front view.
PDB entry 2xqb coloured by chain, side view.
PDB entry 2xqb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering a high-affinity anti-IL-15 antibody: crystal structure reveals an α-helix in VH CDR3 as key component of paratope.
Lowe DC, Gerhardt S, Ward A, Hargreaves D, Anderson M, Ferraro F, Pauptit RA, Pattison DV, Buchanan C, Popovic B, Finch DK, Wilkinson T, Sleeman M, Vaughan TJ, Mallinder PR
J Mol Biol 406 160-75 (2011)
PMID: 21167836

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211192 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-15 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40933 (Residues: 49-162; Coverage: 86%)
Gene name: IL15
Sequence domains: Interleukin 15
Structure domains: Interleukin-15/Interleukin-21
ANTI-IL-15 ANTIBODY Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
ANTI-IL-15 ANTIBODY Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 22.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: C2
Unit cell:
a: 185.186Å b: 43.754Å c: 70.087Å
α: 90° β: 95.95° γ: 90°
R-values:
R R work R free
0.248 0.244 0.318
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

Quick links

Citations

1 review citation

Monoclonal antibody therapy for the treatment of asthma and chronic obstructive pulmonary disease with eosinophilic inflammation.
Nixon et al. (2017)
5 other articles

6 mentions without citation

The structural basis of antibody-antigen recognition.
Sela-Culang et al. (2013)
5 more