› 2xrf

X-ray diffraction
2.3Å resolution

Crystal structure of human uridine phosphorylase 2

Released:
DOI: 10.2210/pdb2xrf/pdb
PDB entry 2xrf coloured by chain, front view.
PDB entry 2xrf coloured by chain, side view.
PDB entry 2xrf coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Welin M, Moche M, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Karlberg T, Kol S, Kotenyova T, Kouznetsova E, Nyman T, Persson C, Schuler H, Schutz P, Siponen MI, Thorsell AG, Tresaugues L, Van Der Berg S, Wahlberg E, Weigelt J, Nordlund P

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131811 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 303 amino acids
Theoretical weight: 34.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95045 (Residues: 23-317; Coverage: 93%)
Gene name: UPP2
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand URA 3 x URA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 95.28Å b: 95.28Å c: 186.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.206
Expression system: Escherichia coli BL21(DE3)

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