› 2xs8

X-ray diffraction
2.5Å resolution

Crystal Structure of ALIX in complex with the SIVagmTan-1 AYDPARKLL Late Domain

Released:
DOI: 10.2210/pdb2xs8/pdb
PDB entry 2xs8 coloured by chain, front view.
PDB entry 2xs8 coloured by chain, side view.
PDB entry 2xs8 coloured by chain, top view.
Source organisms:
Primary publication:
Identification and structural characterization of the ALIX-binding late domains of simian immunodeficiency virus SIVmac239 and SIVagmTan-1.
Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill CP
J Virol 85 632-7 (2011)
PMID: 20962096

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169776 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Programmed cell death 6-interacting protein Chain: A
Molecule details ›
Chain: A
Length: 704 amino acids
Theoretical weight: 78.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WUM4 (Residues: 1-698; Coverage: 80%)
Gene names: AIP1, ALIX, KIAA1375, PDCD6IP
Sequence domains:
Structure domains:
p6-gag Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 2 KDa
Source organism: Simian immunodeficiency virus
Expression system: Not provided
UniProt:
  • Canonical: Q02843 (Residues: 467-481; Coverage: 3%)
Gene name: gag

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 145.542Å b: 99.076Å c: 72.594Å
α: 90° β: 106.87° γ: 90°
R-values:
R R work R free
0.207 0.204 0.261
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

8 review citations

HIV-1 assembly, budding, and maturation.
Sundquist et al. (2012)
7 more

2 mentions without citation

Proline-rich regions and motifs in trafficking: from ESCRT interaction to viral exploitation.
Ren et al. (2011)
1 more