› 2xsz

X-ray diffraction
3Å resolution

The dodecameric human RuvBL1:RuvBL2 complex with truncated domains II

Released:
DOI: 10.2210/pdb2xsz/pdb
PDB entry 2xsz coloured by chain, front view.
PDB entry 2xsz coloured by chain, side view.
PDB entry 2xsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex.
Gorynia S, Bandeiras TM, Pinho FG, McVey CE, Vonrhein C, Round A, Svergun DI, Donner P, Matias PM, Carrondo MA
J Struct Biol 176 279-91 (2011)
PMID: 21933716

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195084 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RuvB-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 367 amino acids
Theoretical weight: 40.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y265 (Residues: 2-456; Coverage: 77%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
Structure domains:
RuvB-like 2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 378 amino acids
Theoretical weight: 42.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y230 (Residues: 2-463; Coverage: 78%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 6 x ATP
1 modified residue:
Ligand MSE 72 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 111.824Å b: 187.925Å c: 244.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Cellular consequences of arginine methylation.
Lorton et al. (2019)
10 more

15 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
14 more