› 2xtj

X-ray diffraction
2.7Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211609 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Proprotein convertase subtilisin/kexin type 9 Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 33.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NBP7 (Residues: 153-451; Coverage: 45%)
Gene names: NARC1, PCSK9, PSEC0052
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
FAB FROM A HUMAN MONOCLONAL ANTIBODY, 1D05 Chain: B
Molecule details ›
Chain: B
Length: 107 amino acids
Theoretical weight: 11.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB FROM A HUMAN MONOCLONAL ANTIBODY, 1D05 Chain: C
Molecule details ›
Chain: C
Length: 106 amino acids
Theoretical weight: 11.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB FROM A HUMAN MONOCLONAL ANTIBODY, 1D05 Chain: D
Molecule details ›
Chain: D
Length: 131 amino acids
Theoretical weight: 14.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB FROM A HUMAN MONOCLONAL ANTIBODY, 1D05 Chain: E
Molecule details ›
Chain: E
Length: 124 amino acids
Theoretical weight: 13.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Proprotein convertase subtilisin/kexin type 9 Chain: P
Molecule details ›
Chain: P
Length: 114 amino acids
Theoretical weight: 12.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NBP7 (Residues: 53-152; Coverage: 15%)
Gene names: NARC1, PCSK9, PSEC0052
Sequence domains: Peptidase inhibitor I9
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 66.589Å b: 67.834Å c: 250.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

36 review citations

The biology and therapeutic targeting of the proprotein convertases.
Seidah et al. (2012)
35 more

9 mentions without citation

Reliable B cell epitope predictions: impacts of method development and improved benchmarking.
Kringelum et al. (2012)
8 more