› 2xub

X-ray diffraction
2.8Å resolution

Human RPC62 subunit structure

Released:
DOI: 10.2210/pdb2xub/pdb
PDB entry 2xub coloured by chain, front view.
PDB entry 2xub coloured by chain, side view.
PDB entry 2xub coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation.
Lefèvre S, Dumay-Odelot H, El-Ayoubi L, Budd A, Legrand P, Pinaud N, Teichmann M, Fribourg S
Nat Struct Mol Biol 18 352-8 (2011)
PMID: 21358628

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase III subunit RPC3 Chain: A
Molecule details ›
Chain: A
Length: 534 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BUI4 (Residues: 1-534; Coverage: 100%)
Gene name: POLR3C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 161.79Å b: 69.38Å c: 75.4Å
α: 90° β: 113.2° γ: 90°
R-values:
R R work R free
0.19 0.188 0.23
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Conservation between the RNA polymerase I, II, and III transcription initiation machineries.
Vannini et al. (2012)
7 more

5 mentions without citation

Molecular structures of unbound and transcribing RNA polymerase III.
Hoffmann et al. (2015)
4 more