› 2xuu

X-ray diffraction
1.8Å resolution

Crystal structure of a DAP-kinase 1 mutant

Released:
DOI: 10.2210/pdb2xuu/pdb
PDB entry 2xuu coloured by chain, front view.
PDB entry 2xuu coloured by chain, side view.
PDB entry 2xuu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A PEF/Y substrate recognition and signature motif plays a critical role in DAPK-related kinase activity.
Temmerman K, de Diego I, Pogenberg V, Simon B, Jonko W, Li X, Wilmanns M
Chem Biol 21 264-73 (2014)
PMID: 24440081

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 38.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53355 (Residues: 1-334; Coverage: 23%)
Gene names: DAPK, DAPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand SO4 3 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 49.74Å b: 84.43Å c: 86.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Kinase Atlas: Druggability Analysis of Potential Allosteric Sites in Kinases.
Yueh et al. (2019)
4 other articles

1 mention without citation

Overview of refinement procedures within REFMAC5: utilizing data from different sources.
Kovalevskiy et al. (2018)