› 2xwt

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE TSH RECEPTOR IN COMPLEX WITH A BLOCKING TYPE TSHR AUTOANTIBODY

Released:
DOI: 10.2210/pdb2xwt/pdb
PDB entry 2xwt coloured by chain, front view.
PDB entry 2xwt coloured by chain, side view.
PDB entry 2xwt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the TSH receptor (TSHR) bound to a blocking-type TSHR autoantibody.
Sanders P, Young S, Sanders J, Kabelis K, Baker S, Sullivan A, Evans M, Clark J, Wilmot J, Hu X, Roberts E, Powell M, Núñez Miguel R, Furmaniak J, Rees Smith B
J Mol Endocrinol 46 81-99 (2011)
PMID: 21247981

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210697 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
THYROID BLOCKING HUMAN AUTOANTIBODY K1-70 HEAVY CHAIN Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: hybrid
Structure domains: Immunoglobulins
THYROID BLOCKING HUMAN AUTOANTIBODY K1-70 LIGHT CHAIN Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 22.6 KDa
Source organism: Homo sapiens
Expression system: hybrid
Structure domains: Immunoglobulins
Thyrotropin receptor Chain: C
Molecule details ›
Chain: C
Length: 239 amino acids
Theoretical weight: 26.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P16473 (Residues: 22-260; Coverage: 32%)
Gene names: LGR3, TSHR
Sequence domains: BspA type Leucine rich repeat region (6 copies)
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 83.02Å b: 89.3Å c: 101.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.227
Expression systems:
  • hybrid
  • Trichoplusia ni

Quick links

Citations

28 review citations

Mechanisms of Autoantibody-Induced Pathology.
Ludwig et al. (2017)
27 more

31 mentions without citation

Novel insights on thyroid-stimulating hormone receptor signal transduction.
Kleinau et al. (2013)
30 more