› 2xwu

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF IMPORTIN 13 - UBC9 COMPLEX

Released:
DOI: 10.2210/pdb2xwu/pdb
PDB entry 2xwu coloured by chain, front view.
PDB entry 2xwu coloured by chain, side view.
PDB entry 2xwu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation.
Grünwald M, Bono F
EMBO J 30 427-38 (2011)
PMID: 21139563

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131772 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SUMO-conjugating enzyme UBC9 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63279 (Residues: 1-158; Coverage: 100%)
Gene names: UBC9, UBCE9, UBE2I
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
Importin-13 Chain: B
Molecule details ›
Chain: B
Length: 963 amino acids
Theoretical weight: 108.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94829 (Residues: 1-963; Coverage: 100%)
Gene names: IPO13, KIAA0724, RANBP13
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 68.7Å b: 126.8Å c: 184Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.268
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
4 more

9 mentions without citation

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
8 more