› 2xzo

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187807 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Regulator of nonsense transcripts 1 Chain: A
Molecule details ›
Chain: A
Length: 623 amino acids
Theoretical weight: 70.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92900 (Residues: 295-925; Coverage: 55%)
Gene names: KIAA0221, RENT1, UPF1
Sequence domains:
Structure domains:
5'-R(*UP*UP*UP*UP*UP*UP*UP)-3' Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand 1PE 3 x 1PE
Ligand ADP 1 x ADP
Ligand ALF 1 x ALF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 77.066Å b: 101.896Å c: 112.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

52 review citations

Nonsense-mediated mRNA decay: an intricate machinery that shapes transcriptomes.
Lykke-Andersen et al. (2015)
51 more

20 mentions without citation

No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors.
Mailliot et al. (2022)
19 more