› 2y0i

X-ray diffraction
2.28Å resolution

FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH TANKYRASE-2 (TNKS2) FRAGMENT PEPTIDE (21-MER)

Released:
DOI: 10.2210/pdb2y0i/pdb
PDB entry 2y0i coloured by chain, front view.
PDB entry 2y0i coloured by chain, side view.
PDB entry 2y0i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains.
Yang M, Chowdhury R, Ge W, Hamed RB, McDonough MA, Claridge TD, Kessler BM, Cockman ME, Ratcliffe PJ, Schofield CJ
FEBS J 278 1086-97 (2011)
PMID: 21251231

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2) 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190553 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:
Poly [ADP-ribose] polymerase tankyrase-2 Chain: S
Molecule details ›
Chain: S
Length: 21 amino acids
Theoretical weight: 2.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H2K2 (Residues: 538-558; Coverage: 2%)
Gene names: PARP5B, TANK2, TNKL, TNKS2

Ligands and Environments

4 bound ligands:
Ligand FE2 1 x FE2
Ligand AKG 1 x AKG
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 86.49Å b: 86.49Å c: 146.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

Vitamin C: the known and the unknown and Goldilocks.
Padayatty et al. (2016)
20 more

3 mentions without citation

Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains.
Yang et al. (2011)
2 more