› 2y0m

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN DOSAGE COMPENSATION FACTORS MSL1 AND MOF

Released:
DOI: 10.2210/pdb2y0m/pdb
PDB entry 2y0m coloured by chain, front view.
PDB entry 2y0m coloured by chain, side view.
PDB entry 2y0m coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for MOF and MSL3 recruitment into the dosage compensation complex by MSL1.
Kadlec J, Hallacli E, Lipp M, Holz H, Sanchez-Weatherby J, Cusack S, Akhtar A
Nat Struct Mol Biol 18 142-9 (2011)
PMID: 21217699

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180145 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase KAT8 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H7Z6 (Residues: 174-458; Coverage: 62%)
Gene names: KAT8, MOF, MYST1, PP7073
Sequence domains:
Structure domains:
Male-specific lethal 1 homolog Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6PDM1 (Residues: 470-540; Coverage: 12%)
Gene names: Msl1, Msl1l1
Sequence domains: PEHE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
Ligand ZN 1 x ZN
2 modified residues:
Ligand ALY 1 x ALY
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I4122
Unit cell:
a: 180.94Å b: 180.94Å c: 80.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Dosage compensation in Drosophila melanogaster: epigenetic fine-tuning of chromosome-wide transcription.
Conrad et al. (2012)
11 more

4 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
3 more