› 2y1l

X-ray diffraction
1.8Å resolution

Caspase-8 in Complex with DARPin-8.4

Released:
DOI: 10.2210/pdb2y1l/pdb
PDB entry 2y1l coloured by chain, front view.
PDB entry 2y1l coloured by chain, side view.
PDB entry 2y1l coloured by chain, top view.
Source organisms:
Entry authors: Barandun J, Schroeder T, Mittl PRE, Grutter MG

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163346 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Caspase-8 subunit p18 Chains: A, C
Molecule details ›
Chains: A, C
Length: 159 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14790 (Residues: 218-374; Coverage: 33%)
Gene names: CASP8, MCH5
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Caspase-8 subunit p10 Chains: B, D
Molecule details ›
Chains: B, D
Length: 104 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14790 (Residues: 376-479; Coverage: 22%)
Gene names: CASP8, MCH5
Sequence domains: Caspase domain
Structure domains: Caspase-like
DARPIN-8.4 Chains: E, F
Molecule details ›
Chains: E, F
Length: 169 amino acids
Theoretical weight: 18.22 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Structure domains: Ankyrin repeat-containing domain
AC-IETD-CHO Chains: G, H
Molecule details ›
Chains: G, H
Length: 4 amino acids
Theoretical weight: 476 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 61Å b: 81.6Å c: 163.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12
  • Not provided

Quick links