› 2y1z

X-ray diffraction
2.5Å resolution

Human alphaB Crystallin ACD R120G

Released:
DOI: 10.2210/pdb2y1z/pdb
PDB entry 2y1z coloured by chain, front view.
PDB entry 2y1z coloured by chain, side view.
PDB entry 2y1z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of R120G disease mutant of human αB-crystallin domain dimer shows closure of a groove.
Clark AR, Naylor CE, Bagnéris C, Keep NH, Slingsby C
J Mol Biol 408 118-34 (2011)
PMID: 21329698

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-crystallin B chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02511 (Residues: 67-157; Coverage: 52%)
Gene names: CRYA2, CRYAB, HSPB5
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand MRD 3 x MRD
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 60.73Å b: 60.73Å c: 100.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.173 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Large potentials of small heat shock proteins.
Mymrikov et al. (2011)
16 more

7 mentions without citation

Evolution of crystallins for a role in the vertebrate eye lens.
Slingsby et al. (2013)
6 more