› 2yb6

X-ray diffraction
1.5Å resolution

Native human Rad6

Released:
DOI: 10.2210/pdb2yb6/pdb
PDB entry 2yb6 coloured by chain, front view.
PDB entry 2yb6 coloured by chain, side view.
PDB entry 2yb6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6.
Hibbert RG, Huang A, Boelens R, Sixma TK
Proc Natl Acad Sci U S A 108 5590-5 (2011)
PMID: 21422291

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 B Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63146 (Residues: 1-152; Coverage: 100%)
Gene names: RAD6B, UBCH1, UBE2B
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

2 bound ligands:
Ligand BME 1 x BME
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P43212
Unit cell:
a: 48.05Å b: 48.05Å c: 124.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

The ubiquitin code.
Komander et al. (2012)
26 more

12 mentions without citation

E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6.
Hibbert et al. (2011)
11 more