› 2yb8

X-ray diffraction
2.3Å resolution

Crystal structure of Nurf55 in complex with Su(z)12

Released:
DOI: 10.2210/pdb2yb8/pdb
PDB entry 2yb8 coloured by chain, front view.
PDB entry 2yb8 coloured by chain, side view.
PDB entry 2yb8 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Histone methylation by PRC2 is inhibited by active chromatin marks.
Schmitges FW, Prusty AB, Faty M, Stützer A, Lingaraju GM, Aiwazian J, Sack R, Hess D, Li L, Zhou S, Bunker RD, Wirth U, Bouwmeester T, Bauer A, Ly-Hartig N, Zhao K, Chan H, Gu J, Gut H, Fischle W, Müller J, Thomä NH
Mol Cell 42 330-41 (2011)
PMID: 21549310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173198 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein Su(z)12 Chain: A
Molecule details ›
Chain: A
Length: 13 amino acids
Theoretical weight: 1.6 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NJG9 (Residues: 79-91; Coverage: 1%)
Gene names: CG8013, Su(z)12
Chromatin assembly factor 1 p55 subunit Chain: B
Molecule details ›
Chain: B
Length: 422 amino acids
Theoretical weight: 47.76 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24572 (Residues: 1-418; Coverage: 97%)
Gene names: CG4236, Caf1, Caf1-55
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 53.031Å b: 87.189Å c: 99.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.198
Expression system: Trichoplusia ni

Quick links

Citations

154 review citations

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Simon et al. (2013)
153 more

9 mentions without citation

EZH2: biology, disease, and structure-based drug discovery.
Tan et al. (2014)
8 more