› 2yh1

Solution NMR

Model of human U2AF65 tandem RRM1 and RRM2 domains with eight-site uridine binding

Released:
DOI: 10.2210/pdb2yh1/pdb
PDB entry 2yh1 coloured by chain, ensemble of 10 models, front view.
PDB entry 2yh1 coloured by chain, ensemble of 10 models, side view.
PDB entry 2yh1 coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF.
Mackereth CD, Madl T, Bonnal S, Simon B, Zanier K, Gasch A, Rybin V, Valcárcel J, Sattler M
Nature 475 408-11 (2011)
PMID: 21753750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150721 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 21.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 148-342; Coverage: 41%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 55%
Refinement method: MODIFIED ARIA
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

26 review citations

RRM-RNA recognition: NMR or crystallography…and new findings.
Daubner et al. (2013)
25 more

13 mentions without citation

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
12 more