2yhy

X-ray diffraction
1.82Å resolution

Structure of N-Acetylmannosamine kinase in complex with N- acetylmannosamine and ADP

Released:

Function and Biology Details

Reactions catalysed:
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate
UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-195078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 36.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y223 (Residues: 406-720; Coverage: 44%)
Gene names: GLCNE, GNE, IBM2
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 90.38Å b: 90.38Å c: 101.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.182
Expression system: Escherichia coli BL21(DE3)