› 2yt4

X-ray diffraction
2.6Å resolution

Crystal structure of human DGCR8 core

Released:
DOI: 10.2210/pdb2yt4/pdb
PDB entry 2yt4 coloured by chain, front view.
PDB entry 2yt4 coloured by chain, side view.
PDB entry 2yt4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human DGCR8 core.
Sohn SY, Bae WJ, Kim JJ, Yeom KH, Kim VN, Cho Y
Nat Struct Mol Biol 14 847-53 (2007)
PMID: 17704815

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microprocessor complex subunit DGCR8 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WYQ5 (Residues: 493-720; Coverage: 30%)
Gene names: C22orf12, DGCR8, DGCRK6, LP4941
Sequence domains: Double-stranded RNA binding motif
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 57.511Å b: 59.985Å c: 70.921Å
α: 90° β: 106.86° γ: 90°
R-values:
R R work R free
0.214 0.214 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
15 more

16 mentions without citation

Molecular mechanisms of RNA interference.
Wilson et al. (2013)
15 more