› 2z5f

X-ray diffraction
2.1Å resolution

Human sulfotransferase Sult1b1 in complex with PAP

Released:
DOI: 10.2210/pdb2z5f/pdb
PDB entry 2z5f coloured by chain, front view.
PDB entry 2z5f coloured by chain, side view.
PDB entry 2z5f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human sulfotransferases SULT1B1 and SULT1C1 complexed with the cofactor product adenosine-3'- 5'-diphosphate (PAP).
Dombrovski L, Dong A, Bochkarev A, Plotnikov AN
Proteins 64 1091-4 (2006)
PMID: 16804942

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1B1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 35.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43704 (Residues: 1-296; Coverage: 100%)
Gene names: ST1B2, SULT1B1, SULT1B2
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 44.813Å b: 77.072Å c: 189.385Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.176 0.241
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regioselective sulfation and glucuronidation of phenolics: insights into the structural basis.
Wu et al. (2011)
4 more

6 mentions without citation

From Steroid and Drug Metabolism to Glycobiology, Using Sulfotransferase Structures to Understand and Tailor Function.
Pedersen et al. (2022)
5 more