› 2z93

X-ray diffraction
2.4Å resolution

Crystal structure of Fab fragment of anti-ciguatoxin antibody 10C9 in complex with CTX3C-ABCD

Released:
DOI: 10.2210/pdb2z93/pdb
PDB entry 2z93 coloured by chain, front view.
PDB entry 2z93 coloured by chain, side view.
PDB entry 2z93 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
How protein recognizes ladder-like polycyclic ethers. Interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9.
Ui M, Tanaka Y, Tsumuraya T, Fujii I, Inoue M, Hirama M, Tsumoto K
J Biol Chem 283 19440-7 (2008)
PMID: 18463096

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234845 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anti-ciguatoxin antibody 10C9 Fab heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 218 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Anti-ciguatoxin antibody 10C9 Fab light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 213 amino acids
Theoretical weight: 23.22 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand END 1 x END
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 80.725Å b: 45.41Å c: 122.371Å
α: 90° β: 98.9° γ: 90°
R-values:
R R work R free
0.297 0.297 0.34
Expression system: Not provided

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Citations

5 review citations

Update on methodologies available for ciguatoxin determination: perspectives to confront the onset of ciguatera fish poisoning in Europe.
Caillaud et al. (2010)
4 more