› 2zjy

X-ray diffraction
2.8Å resolution

Structure of the K349P mutant of Gi alpha 1 subunit bound to ALF4 and GDP

Released:
DOI: 10.2210/pdb2zjy/pdb
PDB entry 2zjy coloured by chain, front view.
PDB entry 2zjy coloured by chain, side view.
PDB entry 2zjy coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Morikawa T, Muroya A, Sugio S, Wakamatsu K, Kohno T

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 1-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ALF 1 x ALF
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3221
Unit cell:
a: 79.375Å b: 79.375Å c: 105.297Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.26
Expression system: Escherichia coli

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