› 2zjz

X-ray diffraction
2.6Å resolution

Structure of the K349P mutant of Gi alpha 1 subunit bound to GDP

Released:
DOI: 10.2210/pdb2zjz/pdb
PDB entry 2zjz coloured by chain, front view.
PDB entry 2zjz coloured by chain, side view.
PDB entry 2zjz coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Morikawa T, Muroya A, Sugio S, Wakamatsu K, Kohno T

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 40.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 1-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P21212
Unit cell:
a: 101.38Å b: 164.07Å c: 53.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.282
Expression system: Escherichia coli

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