› 2zko

X-ray diffraction
1.7Å resolution

Structural basis for dsRNA recognition by NS1 protein of human influenza virus A

Released:
DOI: 10.2210/pdb2zko/pdb
PDB entry 2zko coloured by chain, front view.
PDB entry 2zko coloured by chain, side view.
PDB entry 2zko coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structural basis for dsRNA recognition by NS1 protein of influenza A virus.
Cheng A, Wong SM, Yuan YA
Cell Res 19 187-95 (2009)
PMID: 18813227

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137079 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 73 amino acids
Theoretical weight: 8.31 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03496 (Residues: 1-70; Coverage: 30%)
Gene name: NS
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: S15/NS1, RNA-binding
RNA (5'-R(P*AP*GP*AP*CP*AP*GP*CP*AP*UP*UP*AP*UP*GP*CP*UP*GP*UP*CP*UP*UP*U)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 nucleotides
Theoretical weight: 6.65 KDa
Source organism: Influenza A virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 60.707Å b: 57.218Å c: 83.709Å
α: 90° β: 105.95° γ: 90°
R-values:
R R work R free
0.191 0.189 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Structures of influenza A proteins and insights into antiviral drug targets.
Das et al. (2010)
16 more

28 mentions without citation

Strategies of highly pathogenic RNA viruses to block dsRNA detection by RIG-I-like receptors: hide, mask, hit.
Zinzula et al. (2013)
27 more