› 2a3h

X-ray diffraction
2Å resolution

CELLOBIOSE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHERANS AT 2.0 A RESOLUTION

Released:
DOI: 10.2210/pdb2a3h/pdb
PDB entry 2a3h coloured by chain, front view.
PDB entry 2a3h coloured by chain, side view.
PDB entry 2a3h coloured by chain, top view.
Source organism: Salipaludibacillus agaradhaerens
Primary publication:
Structure of the Bacillus agaradherans family 5 endoglucanase at 1.6 A and its cellobiose complex at 2.0 A resolution.
Davies GJ, Dauter M, Brzozowski AM, Bjørnvad ME, Andersen KV, Schülein M
Biochemistry 37 1926-32 (1998)
PMID: 9485319

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase 5A Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.65 KDa
Source organism: Salipaludibacillus agaradhaerens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: O85465 (Residues: 30-329; Coverage: 80%)
Gene name: cel5A
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 54.71Å b: 69.57Å c: 77.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.137 0.19
Expression system: Bacillus subtilis

Quick links

Citations

4 review citations

Hyaluronidases: their genomics, structures, and mechanisms of action.
Stern et al. (2006)
3 more

2 mentions without citation

Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5).
Aspeborg et al. (2012)
1 more