› 2a3z

X-ray diffraction
2.08Å resolution

Ternary complex of the WH2 domain of WASP with Actin-DNAse I

Released:
DOI: 10.2210/pdb2a3z/pdb
PDB entry 2a3z coloured by chain, front view.
PDB entry 2a3z coloured by chain, side view.
PDB entry 2a3z coloured by chain, top view.
Source organisms:
Primary publication:
Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly.
Chereau D, Kerff F, Graceffa P, Grabarek Z, Langsetmo K, Dominguez R
Proc Natl Acad Sci U S A 102 16644-9 (2005)
PMID: 16275905

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-132781 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
Actin nucleation-promoting factor WAS Chain: C
Molecule details ›
Chain: C
Length: 29 amino acids
Theoretical weight: 3.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42768 (Residues: 430-458; Coverage: 6%)
Gene names: IMD2, WAS
Sequence domains: WH2 motif

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand CA 2 x CA
Ligand ATP 1 x ATP
Ligand GOL 3 x GOL
Ligand MG 1 x MG
Ligand FMT 1 x FMT
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 153.27Å b: 41.798Å c: 119.418Å
α: 90° β: 108.68° γ: 90°
R-values:
R R work R free
0.159 0.153 0.21
Expression system: Not provided

Quick links

Citations

35 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
34 more

28 mentions without citation

Actin structure and function.
Dominguez et al. (2011)
27 more