› 2a4x

X-ray diffraction
1.4Å resolution

Crystal Structure Of Mitomycin C-Binding Protein Complexed with Metal-Free Bleomycin A2

Released:
DOI: 10.2210/pdb2a4x/pdb
PDB entry 2a4x coloured by chain, front view.
PDB entry 2a4x coloured by chain, side view.
PDB entry 2a4x coloured by chain, top view.
Source organism: Streptomyces caespitosus
Primary publication:
The mitomycin C (MMC)-binding protein from MMC-producing microorganisms protects from the lethal effect of bleomycin: crystallographic analysis to elucidate the binding mode of the antibiotic to the protein.
Danshiitsoodol N, de Pinho CA, Matoba Y, Kumagai T, Sugiyama M
J Mol Biol 360 398-408 (2006)
PMID: 16756991

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.47 KDa
Source organism: Streptomyces caespitosus
Expression system: Escherichia coli
UniProt:
  • Canonical: O05205 (Residues: 1-130; Coverage: 100%)
Gene name: mrd
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
Ligand BLM 1 x BLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 47.248Å b: 52.405Å c: 107.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.264
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Endophytic bacteria: a new source of bioactive compounds.
Singh et al. (2017)
1 more