› 2a5v

X-ray diffraction
2.2Å resolution

Crystal structure of M. tuberculosis beta carbonic anhydrase, Rv3588c, tetrameric form

Released:
DOI: 10.2210/pdb2a5v/pdb
PDB entry 2a5v coloured by chain, front view.
PDB entry 2a5v coloured by chain, side view.
PDB entry 2a5v coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural mechanics of the pH-dependent activity of beta-carbonic anhydrase from Mycobacterium tuberculosis.
Covarrubias AS, Bergfors T, Jones TA, Högbom M
J Biol Chem 281 4993-9 (2006)
PMID: 16321983

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 213 amino acids
Theoretical weight: 22.57 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPJ9 (Residues: 2-207; Coverage: 100%)
Gene names: Rv3588c, canB, cynT, mtcA2
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand SCN 4 x SCN
Ligand ZN 11 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 67.884Å b: 70.302Å c: 84.361Å
α: 90° β: 93.41° γ: 90°
R-values:
R R work R free
0.165 0.162 0.214
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Carbonic anhydrase inhibitors.
Supuran CT. (2010)
11 more

7 mentions without citation

Mycobacterium tuberculosisβ-Carbonic Anhydrases: Novel Targets for Developing Antituberculosis Drugs.
Aspatwar et al. (2019)
6 more