› 2aao

X-ray diffraction
2Å resolution

Regulatory apparatus of Calcium Dependent protein kinase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb2aao/pdb
PDB entry 2aao coloured by chain, front view.
PDB entry 2aao coloured by chain, side view.
PDB entry 2aao coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure of the regulatory apparatus of a calcium-dependent protein kinase (CDPK): a novel mode of calmodulin-target recognition.
Chandran V, Stollar EJ, Lindorff-Larsen K, Harper JF, Chazin WJ, Dobson CM, Luisi BF, Christodoulou J
J Mol Biol 357 400-10 (2006)
PMID: 16430916

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-dependent protein kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 19.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06850 (Residues: 428-593; Coverage: 27%)
Gene names: AK1, At5g04870, CPK1, MUK11.19
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 9 x CA
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 35.968Å b: 88.154Å c: 117.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Breaking the code: Ca2+ sensors in plant signalling.
DeFalco et al. (2010)
8 more

6 mentions without citation

The Arabidopsis Calcium-Dependent Protein Kinases (CDPKs) and Their Roles in Plant Growth Regulation and Abiotic Stress Responses.
Shi et al. (2018)
5 more