› 2ad9

Solution NMR

Solution structure of Polypyrimidine Tract Binding protein RBD1 complexed with CUCUCU RNA

Released:
DOI: 10.2210/pdb2ad9/pdb
PDB entry 2ad9 coloured by chain, ensemble of 20 models, front view.
PDB entry 2ad9 coloured by chain, ensemble of 20 models, side view.
PDB entry 2ad9 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH
Science 309 2054-7 (2005)
PMID: 16179478

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150781 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polypyrimidine tract-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26599 (Residues: 49-146; Coverage: 18%)
Gene names: PTB, PTBP1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
5'-R(*CP*UP*CP*UP*CP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

54 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
53 more

22 mentions without citation

Biomolecular Condensates in the Nucleus.
Sabari et al. (2020)
21 more