› 2aeq

X-ray diffraction
3Å resolution

An epidemiologically significant epitope of a 1998 influenza virus neuraminidase forms a highly hydrated interface in the NA-antibody complex.

Released:
DOI: 10.2210/pdb2aeq/pdb
PDB entry 2aeq coloured by chain, front view.
PDB entry 2aeq coloured by chain, side view.
PDB entry 2aeq coloured by chain, top view.
Source organisms:
Primary publication:
An epidemiologically significant epitope of a 1998 human influenza virus neuraminidase forms a highly hydrated interface in the NA-antibody complex.
Venkatramani L, Bochkareva E, Lee JT, Gulati U, Graeme Laver W, Bochkarev A, Air GM
J Mol Biol 356 651-63 (2006)
PMID: 16384583

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-211535 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 43.87 KDa
Source organism: Influenza A virus
UniProt:
  • Canonical: Q80DL0 (Residues: 75-469; Coverage: 84%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase
FAB light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig heavy chain Mem5 Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P84751 (Residues: 1-217; Coverage: 92%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NAG 7 x NAG
Ligand MAN 4 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P4212
Unit cell:
a: 159.753Å b: 159.753Å c: 104.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.267 0.312

Quick links

Citations

6 review citations

Influenza neuraminidase.
Air GM. (2012)
5 more

8 mentions without citation

Antibody-Mediated Catalysis in Infection and Immunity.
Bowen et al. (2017)
7 more