› 2aff

Solution NMR

The solution structure of the Ki67FHA/hNIFK(226-269)3P complex

Released:
DOI: 10.2210/pdb2aff/pdb
PDB entry 2aff coloured by chain, ensemble of 100 models, front view.
PDB entry 2aff coloured by chain, ensemble of 100 models, side view.
PDB entry 2aff coloured by chain, ensemble of 100 models, top view.
Source organism: Homo sapiens
Primary publication:
Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67.
Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD
Nat Struct Mol Biol 12 987-93 (2005)
PMID: 16244663

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155402 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferation marker protein Ki-67 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46013 (Residues: 1-120; Coverage: 4%)
Gene name: MKI67
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4
MKI67 FHA domain-interacting nucleolar phosphoprotein Chain: B
Molecule details ›
Chain: B
Length: 44 amino acids
Theoretical weight: 5.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BYG3 (Residues: 226-269; Coverage: 15%)
Gene names: MKI67IP, NIFK, NOPP34
Sequence domains: FHA Ki67 binding domain of hNIFK

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SEP 1 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Multisite protein phosphorylation--from molecular mechanisms to kinetic models.
Salazar et al. (2009)
6 more

12 mentions without citation

Mechanistic insights into phosphoprotein-binding FHA domains.
Liang et al. (2008)
11 more