› 2ako

X-ray diffraction
2.2Å resolution

Crystal structure of Glutamate 5-kinase from Campylobacter jejuni

Released:
DOI: 10.2210/pdb2ako/pdb
PDB entry 2ako coloured by chain, front view.
PDB entry 2ako coloured by chain, side view.
PDB entry 2ako coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Gorman J, Shapiro L, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate = ADP + L-glutamate 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate 5-kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 251 amino acids
Theoretical weight: 28.12 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9PJ29 (Residues: 1-251; Coverage: 100%)
Gene names: Cj0097, proB
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 98.597Å b: 99.409Å c: 124.547Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.221
Expression system: Escherichia coli BL21

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